Subconfluent monolayers
Weband 95% air to obtain subconfluent cell monolayers (70-90% confluency) before use. The cells were treated for 48 h with ethanol and aqueous extracts with concentrations ranging from 250 ng/mL to 8000 ng/mL. The treatment medium was removed and 500 μL of neutral red medium (50 μg/mL) was added to each well. The plate was incubated at 37 °C Web4 Feb 2005 · In the present study, we have focused our analysis on the comparison of the mechanical activity of subconfluent monolayers versus isolated single cells obtained by the addition of HGF. The microdimensional force sensor array (μFSA) is made of elastomeric pillars with known physical and surface chemical properties ( 21 ).
Subconfluent monolayers
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Web6 Aug 1999 · Subconfluent monolayers of ECs (in 100-mm plates) were treated with trypsin, counted, and then washed with PBS. HUVECs (4.0×10 6) or ECV cells (2.0×10 6) were … WebAdditionally, we established monolayers of intestinal epithelial cells (IECs) from human iPS cell-derived intestinal organoids (hiPSOs) 10 and primary human ileum organoids ... Briefly, subconfluent iPS cells were differentiated into definitive endoderm by treating with Roswell Park Memorial Institute (RPMI) 1640 supplemented with 2 mM L ...
Web(A) Subconfluent monolayers of CHO cells were transfected with plasmid DNA in conjunction with pGL2-control vector and harvested at 48 h. 13-gal activity was … WebMonolayers that are particularly difficult to detach can be placed at 37°C to facilitate dispersal. Prewarming of the enzyme solution will also decrease the exposure period. To …
WebOnly subconfluent monolayers of cells were used in all experiments. Fernando J. Reyes-Zurita, Eva E. Rufino-Palomares, Leticia García-Salguero, Juan Peragón, Pedro P. Medina, Andrés Parra, Marta Cascante, José A. Lupiáñez Web2 May 2014 · The Journal of Biological Chemistry May 2, 2014. Abstract. S100B is a prognostic marker for malignant melanoma. Increasing S100B levels are predictive of advancing disease stage, increased ...
WebSubconfluent cultures or monolayers with large gaps between the cells will still influence the impedance spectra (Janshoff et al, 1996), but if parts of the electrode are uncovered the …
WebWe found that isolated apical membranes from confluent MDCK II monolayers display similar mechanical properties as the apical side of living MDCK II cells in a confluent … honeymoon themed hotel roomsWeb4 Jan 2024 · The generation of vesicular stomatitis virus G protein-pseudotyped self-inactivating LV particles was performed by transfecting subconfluent monolayers of HEK293T cells with one of the LV shuttle plasmids and the packaging plasmids psPAX2 (Addgene, plasmid number: 12260) and pLP/VSVG (Thermo Fisher Scientific) at a molar … honeymoon the maldivesWeb1 Aug 2002 · Confluent, sub-confluent and wounded layers were processed for localisation of occludin using a rabbit polyclonal antibody recognising the C-terminal domain of human occludin. Rabbit polyclonal antibody to mouse ZO-1 ( Sheth et al., 1997) and ZO-2 (Zymed) were also used for immunostaining. honeymoon themed hotelsWebHeLa cells were cultured as exponentially growing subconfluent monolayers on 90-mm plates in RPMI 1640 medium (Invitrogen) supplemented with 10% (v/v) fetal calf serum and 2 m m glutamine. CHO-K1 cells were cultured as exponentially growing subconfluent monolayers on 25-cm 2 culture flasks in F-12K medium (Invitrogen) supplemented with … honeymoon themesWeb31 Dec 2004 · We find confluent monolayers of human umbilical vein endothelial cells were quiescent and expressed low levels of cyclooxygenase-2, but expressed cyclooxygenase … honeymoon time word stackWebTransformed fibroblast cells were maintained as subconfluent monolayers, trypsinized, and seeded in 96-well plates (for analytical assays; Fig. 1) or in 384-well white plates (for … honeymoon ti kaye resort \u0026 spa marchWebSubconfluent monolayers of DCs isolated from C57BL/6, 129SVE, and BALB/c mice were infected with 10 PFU/cell of HSV-CD80 or parental virus as described in Materials and … honeymoon tights