WebApr 3, 2024 · Zhou et al. find that association of the rixosome, which degrades RNA, and Polycomb silencing complexes is regulated by enzymatic removal of ubiquitin and the … WebColocalization of the rixosome with Polycomb bodies a, b, Immunoblot validation of siRNA-mediated WDR18 (a) and EZH2 (b) knockdowns. c, ... Haining Zhou. Chad Stein. Tiasha A. …
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WebZhou et al. set out to investigate a possible role for a protein complex called the rixosome in gene-repression pathways in human cells. The authors investigated two enzymatic functions of the rixosome: first, as an endonuclease that cleaves RNA; and, second, as a kinase that attaches phosphate groups to the cleaved molecule, flagging it for WebThe rixosome and PRC1 silencing complexes are associated with deSUMOylating and deubiquitinating enzymes, SENP3 and USP7, respectively. ... Haining Zhou 1 , Wenzhi … hand sanitizer purell ingredients
Colocalization of the rixosome with Polycomb bodies a, b, …
Rixosome subunits (NOL9, WDR18, PELP1, TEX10), PRC2 subunits (EZH2, EED, SUZ12), PRC1 subunits (RING1B, PCGF1-4) and CBX1-8 cDNAs were amplified from human ES cell cDNA library and inserted to pGAD-T7 (Takara, 630442) and pGBK-T7 (Takara, 630443) plasmids for Y2H assays. NOL9 siRNA resistant … See more Y2H budding yeast strain (Takara) was cultured with YEPD+adenine overnight at 30 °C. Yeast cells were collected OD 0.5 by centrifugation at 3,000 rpm for 3 min. Cells were resuspended … See more Small guide RNA was synthesized via in vitro transcription by using MAXIscript T7 transcription kit (ThermoFisher, AM1312). CRISPR–Cas9 protein was purified by the Initiative for Genome … See more HeLa (ATCC, CCL-2), and HEK 293FT (ThermoFisher, R70007) cells were cultured in DMEM containing 10% fetal calf serum. Human … See more For siRNA-mediated knockdown, Lipofectamine RNAiMAX transfection reagent (Invitrogen) and siRNA (200 nM) were used to transfect the cells by following the … See more WebAuthor links open overlay panel Haining Zhou 1 2, Wenzhi Feng 1, Juntao Yu 1, Tiasha A. Shafiq 1, ... The rixosome is a highly conserved complex with essential roles in rRNA … WebHaining Zhou, Wenzhi Feng, Juntao Yu, Tiasha A. Shafiq, Joao A. Paulo, Jiuchun Zhang, Zhenhua Luo, Steven P. Gygi, and Danesh Moazed. 1 Figure S1. ... SENP3 and USP7 … business comunications sheffield