WebThe intergenic control region 1 (IGCR1) in the V H-to-D intergenic region regulates Igh V(D)J recombination in the contexts of developmental order, lineage specificity, and … Web1 jul. 2024 · Intergenic control region 1 (IGCR1), which contains 2 CTCF sites, lies just upstream of the distal D segment DFL16.1 (refs 82,83), and a cluster of 10 CTCF sites …
Frontiers Igh Locus Polymorphism May Dictate Topological Chromatin ...
Web11 feb. 2024 · Here we report that in the absence of intergenic control region 1 (IGCR1) the intronic enhancer (Eµ) associates with the next available CTCF binding site located close to VH81X, via putative heterotypic interactions involving YY1 and CTCF. Figure 1 and S3 show that Eµ loops to an alternate site close to the 3’-most functional VH gene segment, … WebFunctionally, IGCR1 uses CTCF looping/insulator factor-binding elements and, correspondingly, mediates Igh loops containing distant enhancers. IGCR1 promotes normal B-cell development and balances antibody repertoires by inhibiting transcription and rearrangement of D H-proximal V H gene segments and promoting rearrangement of … brandao zerozero
RAG1 and RAG2 Recruitment to WT and IGCR1-Mutated
WebFigure S2.Epigenetic features of WT and IGCR1-mutated IgH alleles-1, related to Figure 1. IGCR1-mutated IgH alleles. Four RAG2-deficient pro-B cell lines containing wild-type IgH alleles (WT), IGCR1- mutated IgH alleles (CBE-/ (1) and (2)), and Eµ-deficient IgH alleles (Eµ-/), were used in chromatin immunoprecipitation (ChIP) assays using anti-H3K4me3, … WebDownload scientific diagram IGCR1 mutations alter VH usage, germline transcription and rearrangement order. a, PCR analyses of indicated VH family rearrangements in pro-B cells from indicated ... Web18 mei 2024 · Figure 1 The Igh locus contains ~100 V H gene segments over an almost 3Mb genomic interval. (A) (Upper panel) Diagram of the Igh locus indicating V H, D, J H, and C H exons and regulatory elements (not to scale). The intronic Eμ and 3′Eα super-enhancers and intergenic control region 1 (IGCR1), composed of two divergent CBEs, … brandao velez