WebProtocol: Prepare: shaking waterbath 37°C Thaw isolation buffer and add collagenase (1.5mg/ml) (Collagenase A, B&M Roche, catalog #103 578) vortex Sterile PBS … WebBackground: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This …
Cell Press: STAR Protocols
WebMeasure Glucose Levels (in mM) Cut right pre ventricle and flush mouse with 5ml of PBS via the left ventricle using a 10 ml syringe and a 27 ga needle. spin blood 8 min at 8000 rpm, transfer plasma into 1.5 ml tube and freeze at -80 °C. Excise subcutaneous and visceral adipose tissue. Mince fat tissue and incubate at 37 °C with mild rocking ... Webprotocol for preparing a primary adipose cell culture, we di-gested freshly harvested mouse epididymal fat pads with crude collagenase at 37 °C for 2 h, followed by extensive … eschools calton primary school
In vitro and ex vivo models of adipocytes American …
WebIn summary, isolation and culture of adipocytes in vivo and gene expression studies will help to understand the mechanisms that control adipocyte function in … WebJun 24, 2024 · A protocol for human bone marrow adipocyte isolation and purification A protocol for human bone marrow adipocyte isolation and purification STAR Protoc. 2024 Jun 24;2 (3):100629. doi: 10.1016/j.xpro.2024.100629. eCollection 2024 Sep 17. Authors Camille Attané 1 2 , David Estève 1 2 , Mohamed Moutahir 1 2 , Nicolas Reina 3 4 , … Web3T3-L1 Differentiation Protocol. Materials. - Dulbeco’s Modified Eagles Medium (DMEM, GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate) - Calf Serum (GibcoBRL-Cat# 10437-028/Lot # 1026566), filter sterilize (0.22 μL filter) before mixing into DMEM. - Isobutylmethylxanthine (IBMX; Sigma I ... eschools calton